The electronic in-line pump (EIP) is a complex system consisting of mechanical, hydraulic, and electromagnetic parts. Experimental study showed that the fuel pressure of the plunger and the fuel drainage of the pressure system after fuel injection could result in fuel pressure fluctuation in the low pressure system. Such fluctuation exhibited pulsating cycle fluctuation as the amplitude rose with the increase of the injection pulse width. The time domain analysis found that the pressure time history curve and injection cylinders corresponded with a one-to-one relationship. By frequency domain analysis, the result was that with the increase of the working cylinder number, the high frequency amplitude gradually increased and the basic frequency amplitude gradually decreased. The conclusion was that through wavelet transformation, the low pressure signal simultaneously moved towards low frequency as the high frequency of the wavelet transformation signal with the working cylinder number increased. Lastly, by using the numerical model, the study investigated the simulation research concerning the relationship of the fluctuation dynamic characteristic in the low pressure system and the fuel injection characteristic of the high pressure system, completing the conclusions obtained by the experimental study.