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In the present study,two new diterpenoid lactones,3-deoxy-andrographoside (1) and 14-deoxy-15-methoxy-andrographolide (2),were isolated from the aerial parts of Andrographis paniculata.Their structures were elucidated by combination of NMR,MS,and chemical methods.The configurations of 1 and 2 were established based on the analysis of ROESY data and single crystal X-ray diffraction experiment.