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目的,观察益气止血中药对脾不统血证模型大鼠前阿黑皮素(POMC)基因表达的影响。方法:以原位杂交结合图像分析的方法检测大脑皮层、海马、下丘脑前阿黑皮素信使核糖核酸(POMC mRNA)的积分光密度和面积。结果:脾不统血证模型大鼠脑组织POMC mRNA的积分光密度和面积均较正常显著增强,益气止血中药复方对各部位POMC的基因表达均有下调作用。结论:益气止血中药复方对各部位POMC的基因表达均有较好的调节作用,表现出单纯用药的协同作用,且其作用也略强于阳性对照药归脾丸。
Objective To observe the effect of Chinese medicine for supplementing Qi and stopping blood on the expression of pre-amelogenin (POMC) gene in the rat model of spleen uncontrolled blood. METHODS: The integrated optical density and area of the cerebral cortex, hippocampus, and hypothalamic POMC mRNA were detected by in situ hybridization and image analysis. Results: The integrated optical density and area of POMC mRNA in the brain of rats with spleen uncontrolled blood stagnation were significantly higher than normal, and the gene expression of POMC in each site had a down-regulation effect. Conclusion: The formula of Qi-qi and blood-stopping Chinese herbal medicine has good regulation effect on gene expression of POMC in various parts, showing the synergistic effect of simple drug use, and its effect is also slightly stronger than the positive control drug Guipi pills.