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目的建立HPLC法测定人血浆中替米沙坦浓度的分析方法。方法色谱柱采用Agilent ZORBAX SB-C18(150mm×4.6mm,5μm),流动相为乙腈:0.03 mol.L-1KH2PO4溶液(47∶55,v/v),流速为1.0mL.min-1,荧光检测激发波长为306nm,发射波长为374nm。结果线性范围为5~1500ng.mL-1,回归方程为Y=0.01852X-0.01655,r=0.9997(n=5);最低定量下限(LLOD)为5ng.mL-1。结论本方法准确、简便,重现性好,适用于替米沙坦的临床药动学研究。
Objective To establish an HPLC method for the determination of telmisartan in human plasma. Methods The column was Agilent ZORBAX SB-C18 (150mm × 4.6mm, 5μm) with a mobile phase of acetonitrile: 0.03mol·L-1KH2PO4 (47:55, v / v) The detection excitation wavelength is 306 nm and the emission wavelength is 374 nm. Results The linear range was 5-1500 ng.mL-1, the regression equation was Y = 0.01852X-0.01655, r = 0.9997 (n = 5), and the lowest limit of quantitation (LLOD) was 5 ng.mL-1. Conclusion The method is accurate, simple and reproducible. It is suitable for clinical pharmacokinetic studies of telmisartan.