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以我国主要推广的优良杂交稻亲本(珍汕97B和9311)为受体亲本,以从国际合作网络所征集的品种资源150多份为供体材料,进行了大规模杂交、3 ̄4次回交,1 ̄2次自交,培育珍汕97B和9311为背景的近等基因导入系近5000多份。通过对导入系材料进行品质、耐盐、耐旱、磷高效、氮高效利用等性状的筛选鉴定,获得了大量目标性状的导入系。通过对品质性状导入系的基因型分析,定位了影响粒型的15个染色体区段(或基因)。同时,构建了一套来源于粳稻日本晴的单片段导入系群体。创建的近等基因导入系(群体)为目标基因的发掘、新品种的培育提供重要的材料基础。
Taking the parents of excellent hybrid rice (Zhenshan 97B and 9311), which are mainly popularized in China, as the recipient parents, more than 150 varieties of cultivars collected from international cooperation networks were used as donor materials for large-scale hybridization and 3 to 4 backcrossing , 1-2 times inbreds, and nearly 5000 inbred lines for cultivating Zhenshan 97B and 9311 as background. Through the screening and identification of traits such as quality, salt tolerance, drought tolerance, high phosphorus efficiency and high nitrogen use efficiency, the introduction system has obtained a large number of target traits. By analyzing the genotypes of the quality traits, 15 chromosome segments (or genes) affecting the grain size were located. At the same time, a set of single-segment-derived lines derived from japonica rice Nipponbare was constructed. The near-isogenic introduction lines (populations) created provide an important material basis for discovering target genes and cultivating new varieties.