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蛋白酶体途径对花粉发育调控具有重要作用,但花粉发育过程中蛋白酶体的分布及其活性的动态变化一直未见报道。蛋白酶体荧光底物结合荧光分光光度计分析表明,蛋白酶体的活性从单核小孢子到具有2个原叶细胞的三细胞花粉逐渐增强,而在成熟花粉中略有下降。免疫荧光标记结合共聚焦显微镜分析表明,蛋白酶体不均匀地分布于细胞质和细胞核中,并在花粉细胞不均等分裂过程中聚集分布于先后产生的2个原叶细胞内。总之,蛋白酶体的活性及其分布在花粉发育过程中存在相关的时空动态变化,表明裸子植物花粉中的蛋白酶体活性及其分布与花粉发育具有相关性,并在原叶细胞的退化过程中起重要作用。
Proteasome pathway plays an important role in the regulation of pollen development. However, the distribution and activity of proteasome in pollen development has not been reported yet. Proteasome fluorescence substrate combined with fluorescence spectrophotometer analysis showed that the activity of proteasome gradually increased from single-core microspore to three-cell pollen with two primary leaf cells, but decreased slightly in mature pollen. Immunofluorescence labeling combined with confocal microscopy showed that the proteasome was unevenly distributed in the cytoplasm and nucleus and aggregated in two successive progenitor cells during the process of uneven division of pollen cells. In conclusion, the proteasome activity and its distribution have some spatiotemporal changes in pollen development, suggesting that proteasomal activity and its distribution in gymnosperm pollen are correlated with pollen development and play an important role in the degeneration of primary leaf cells effect.