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目的 探讨与致癌物亚硝胺代谢激活有关的细胞色素P45 0 (CYP) 2E1基因多型性与胃癌易感性关系。方法 采用病例对照分子流行病学研究方法 ,以聚合酶链反应 限制性片段长度多态性(PCR RFLP)技术 ,对福建省长乐市 92例胃癌病例和 92例正常对照的CYP2E1基因型进行检测。结果 在病例组和对照组中 ,RsaⅠ识别的野生型 (C1/C1)基因频率分别为 6 6 .3%和 48.9% ;DraⅠ识别的野生型 (CC)基因频率分别为 5 7.6 %和 41.3% ,差异均具有显著性 (P <0 .0 5 ) ,即具有RsaⅠ识别的C1/C1基因型或DraⅠ识别的CC基因型个体 ,患胃癌的危险性大 (OR值分别为 2 .0 6和 1.93)。联合分析表明 ,这两个位点的基因型存在某种程度的联合作用 (P <0 .0 5 )。结论 CYP2E1基因多型性可能与胃癌易感性有关。
Objective To investigate the relationship between the polymorphism of cytochrome P45 0 (CYP) 2E1 gene and the susceptibility of gastric cancer to the carcinogenic nitrosamine metabolism. Methods The case-control molecular epidemiology method was used to detect CYP2E1 genotypes in 92 gastric cancer cases and 92 normal controls in Changle City of Fujian Province by polymerase chain reaction restriction fragment length polymorphism (PCR RFLP). Results The frequency of wild-type (C1 / C1) gene recognized by RsaⅠwas 66.3% and 48.9% respectively in case group and control group. The frequency of wild type (CC) gene identified by DraⅠ was 57.6% and 41.3% (P <0.05), that is, individuals with genotype C1 / C1 with RsaI or CC genotypes with DraI had a high risk of developing gastric cancer (OR = 2.06 and 1.93). Joint analysis showed that the two loci genotypes have some degree of combined effect (P <0.05). Conclusion CYP2E1 gene polymorphism may be related to gastric cancer susceptibility.