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[目的]探讨p53突变在脑胶质瘤发生发展中的作用及其与临床病理特征的关系。[方法]利用聚合酶链反应-单链构象多态性分析(PCR鄄SSCP)及LSAB免疫组化法对已确诊的48例脑胶质瘤进行p53基因突变以及蛋白表达的检测。[结果]48例胶质瘤中p53蛋白阳性表达率41.7%(20/48)。在高级别(Ⅲ、Ⅳ级)中p53表达率明显高于低级别(Ⅱ级)的肿瘤,分别是63.2%、27.6%(P<0.05)。PCR鄄SSCP检测发现17例(35.4%)呈现p53基因突变,均位于5～8外显子,突变例数依次为7(41.2%)、1(5.9%)、4(23.5%)、5(29.4%)。两种方法检测的符合率为89.6%(43/48)。p53基因的突变与患者性别、年龄、肿瘤部位及大小无关。[结论]胶质瘤中p53突变多在第5～8外显子,p53基因突变在胶质瘤的发生进展中起重要作用。
[Objective] To investigate the role of p53 mutation in the development of glioma and its relationship with clinicopathological features. [Method] The p53 gene mutation and protein expression in 48 cases of gliomas confirmed by polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SSCP) and LSAB immunohistochemical method. [Results] The positive rate of p53 protein in 48 gliomas was 41.7% (20/48). The positive rate of p53 in high grade (Ⅲ, Ⅳ grade) was significantly higher than that in lower grade (grade Ⅱ), which was 63.2% and 27.6% respectively (P <0.05). The results of PCR-SSCP showed that p53 gene mutations were found in 17 cases (35.4%), all of which were located in exon 5 to exon 7, with mutations of 7 (41.2%), 1 (5.9%), 4 (23.5% 29.4%). The coincidence rate of the two methods was 89.6% (43/48). The mutation of p53 gene has nothing to do with patient’s sex, age, tumor location and size. [Conclusion] The mutation of p53 in glioma is more than exon 5 ~ 8, p53 gene mutation plays an important role in the progress of glioma.