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目的 :探讨Pre_S2 、抗Pre_S2 二对半和HBVDNA对乙型肝炎患者血清学的诊断意义。方法 :用ELISA法和PCR法分别检测 93例乙型肝炎患者和 2 8例健康人血清中的Pre_S2 ,抗Pre_S2 ,二对半和HBVDNA水平。结果 :93例乙肝患者HBsAg、HBeAg、HBcAb阳性 32例 ,HBsAg、HBeAb、HBcAb阳性 39例 ,HBsAg、HBcAb阳性 18例 ,HBcAb阳性 4例。Pre_S2 结果阳性率 6 6 .7% (6 2 / 93) ,Pre_S2 抗体结果阳性率 1.1% (1/ 93) ,HBVDNA结果阳性率40 .9% (38/ 93)。 2 8例健康人二对半 ,五项全阴 16例 ,HBsAb阳性 12例 ,Pre_S2 阳性 1例 ,HBVDNA阳性 1例 ,Pre_S2 抗体阳性 5例占HBsAb阳性 41.7% (5 / 12 ) ,Pre_S2 和HBVDNA相比有明显差异 (P <0 .0 1) ,非HBeAg阳性组中HBVDNA阳性率约低于Pre_S2 。结论 :Pre_S2 ,抗Pre_S2 ,HBVDNA和二对半在乙型肝炎血清学诊断中显示 ,Pre_S2 优于HBVDNA和二对半。Pre_S2 抗体出现早于抗HBs和抗HBe ,抗Pre_S2 阳性检出率较低 ,可能同乙型肝炎疫苗中抗Pre_S2 含量低有关。
Objective: To investigate the serological diagnostic significance of Pre_S2, Pre_S2 two-and-a-half and HBVDNA in hepatitis B patients. Methods: Serum Pre_S2, anti-Pre_S2, two-and-a-half, and HBVDNA levels in 93 hepatitis B patients and 28 healthy controls were detected by ELISA and PCR respectively. Results: Of the 93 patients with hepatitis B, 32 were positive for HBsAg, HBeAg and HBcAb, 39 were HBsAg, HBeAb and HBcAb, 18 were HBsAg and HBcAb, and 4 were HBcAb. The positive rate of Pre_S2 was 66.7% (62/93), the positive rate of Pre_S2 was 1.1% (1/93), and the positive rate of HBVDNA was 40.9% (38/93). There were 16 healthy HBsAb positive cases, 12 positive cases of HBsAb, 1 positive case of Pre_S2, 1 positive case of HBVDNA, 5 cases positive of PreS2 positive case (41.7% (P <0.01), the positive rate of HBVDNA in non-HBeAg positive group was lower than that of Pre_S2. CONCLUSIONS: Pre_S2, anti-Pre_S2, HBVDNA and two-and-a-half serological diagnosis of hepatitis B show that Pre_S2 is superior to HBVDNA and two-and-a-half pairs. Pre_S2 antibody appeared earlier than anti-HBs and anti-HBe, anti-Pre_S2 positive detection rate is low, may be associated with low anti-Pre_S2 hepatitis B vaccine.