目的　采用α CD2 8单抗增强α CD3单抗刺激的肿瘤特异性T细胞的杀瘤活性。方法　采用 2种方案培养FBL 3免疫的小鼠脾细胞。 (1)使用α CD3单抗 48h后 ,加入α CD3单抗和 2 0U/mlrIL 2 (α CD3 +IL 2组 ) ;(2 )α CD3单抗和α CD2 8单抗同时使用 48h后 ,加入α CD3单抗、α CD2 8单抗和 2 0U/mlrIL 2 (α CD3+α CD2 8+IL 2组 )。3H TdR掺入法检测两组效应细胞的增殖水平。标准 4h 51Cr释放法检测两组效应细胞的杀伤活性。在培养第 12天 ,每只荷瘤小鼠过继转输 1× 10 7CTL ,观察小鼠的存活期。结果　α CD3 +IL 2组、α CD3+α CD2 8+IL 2组细胞的3H TdR掺入量分别为 8.36× 10 4 、1.31× 10 5,后者明显高于前者。在培养 12d时 ,效靶比为 12 .5∶1时 ,α CD3+IL 2组细胞对FBL 3的特异性杀伤活性为 35 .6 % ,α CD3 +α CD2 8+IL 2组细胞对FBL 3的特异性杀伤活性为 49.5 %。分别过继转输两组效应细胞治疗荷瘤小鼠 ,荷瘤小鼠的平均存活期分别为 16 .7和 2 0 .8d。结论　协同刺激信号α CD2 8单抗增强肿瘤特异性CTL体内外的杀瘤活性 OBJECTIVE: To enhance the cytotoxicity of tumor-specific T cells stimulated by α CD3 monoclonal antibody with α CD2 8 monoclonal antibody. Methods Two kinds of regimens were used to culture mouse spleen cells immunized with FBL 3. (1) α CD3 monoclonal antibody and 20 U / ml rIL 2 (α CD3 + IL 2 group) were added after 48h with α CD3 monoclonal antibody; (2) After 48h simultaneous use of α CD3 monoclonal antibody and α CD2 8 monoclonal antibody, α CD3 mAb, α CD2 8 mAb and 20 U / ml rIL 2 (α CD3 + α CD2 8 + IL 2 group). 3H TdR incorporation assay was used to detect the proliferation of two effector cells. Standard 4h 51Cr release assay of two groups of effector cell killing activity. On day 12 of culture, 1 × 10 7 CTL of each tumor-bearing mice was adoptively transferred to observe the survival of mice. Results 3H TdR incorporation in α CD3 + IL 2 group and α CD3 + α CD2 8 + IL 2 group was 8.36 × 10 4 and 1.31 × 10 5, respectively, which was significantly higher than that of the former. The specific killing activity of FBL 3 in α CD3 + IL 2 cells was 35.6% at 12 d after culture at 12 d, and the effect of α CD3 + α CD2 8 + IL 2 on FBL 3 specific killing activity was 49.5%. Respectively adoptive transfer of two groups of effector cells in tumor-bearing mice, the average survival of tumor-bearing mice were 16.7 and 20.8 days. Conclusions Costimulatory signal α CD2 8 mAb enhances the cytotoxic activity of tumor-specific CTL in vivo and in vitro