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目的为了深入研究稀土配合物的抗肿瘤活性,将稀土配合物开发成抗癌新药,本实验对钐、钇配合物抑制胃癌细胞BGC-823细胞增殖的活性作用及其机制作了探讨。方法磺酰罗丹明B(SRB)法测定钇、钐配合物及硝酸盐对人胃癌细胞株BGC-83增殖的影响;观察稀土配合物诱导BGC-823细胞凋亡,采用流式细胞仪检测稀土配合物对BGC-823细胞周期的影响,运用彗星微凝胶单细胞电泳观察其对BGC-823细胞DNA剪切作用。结果钇、钐配合物明显抑制BGC-823的增殖,并能诱导BGC-823凋亡;经不同浓度钐、钇配合物处理BGC-823细胞的细胞周期发生明显变化并损伤细胞的DNA。结论稀土配合物的体外抗肿瘤活性可能是在5-Fu配体的细胞毒性基础上,稀土离子发挥了协同作用;钐、钇稀土配合物可能通过将胃癌BGC-823细胞阻滞于G0/G1期或直接切断肿瘤细胞DNA诱导细胞凋亡,从而发挥其抗肿瘤活性作用。
In order to further study the anti-tumor activity of rare earth complexes and to develop new rare earth complexes into anticancer drugs, we investigated the inhibitory effect of samarium and yttrium complexes on the proliferation of BGC-823 cells and its mechanism. Methods The effects of yttrium and samarium complexes and nitrate on the proliferation of human gastric cancer cell line BGC-83 were determined by sulforhodamine B (SRB) method. The apoptosis of BGC-823 cells was observed by using rare earth complexes. The contents of rare earth The effect of the complex on the cell cycle of BGC-823 cells was observed by using single cell gel electrophoresis of comet microgel. Results The complexes of yttrium and samarium significantly inhibited the proliferation of BGC-823 cells and induced the apoptosis of BGC-823 cells. The cell cycle of BGC-823 cells treated with different concentrations of samarium and yttrium complexes significantly changed and damaged the DNA of cells. CONCLUSION: The anti-tumor activity of rare earth complexes in vitro may be based on the cytotoxicity of 5-Fu ligands, and the rare earth ions play a synergistic effect. The samarium and yttrium rare earth complexes may inhibit the growth of BGC-823 cells in G0 / G1 Period or directly cut off the DNA of tumor cells to induce apoptosis, thus exerting antitumor activity.