论文部分内容阅读
目的:了解VP的耐药性,发现流行优势型,为追溯病原和治疗提供依据。方法:VP分离海产品采用筛检方法,病人采用GB方法;用诊断血清进行血清分型,用PFGF进行基因分型;用K-B法进行耐药性测定。结果:1040株VP分为10个血清型,分型率为94.64%,海产品VP的流行优势型为0:6群和0:5群,病人VP为0:3群;VP对氨苄西林耐药率较高,达92.64%,未检出多重耐药菌;按PFGE带型变化病人VP分为12型,海产品VP为29型,1型为流行优势型。结论:宁波地区海产品VP的血清型和PFGE型较分散,病人VP则反之,流行优势株上两者有明显差别,提示我们可用血清和PFGE分型,对其追踪与溯源;VP对青霉素类耐药性较高,是否与养殖广泛使用有关需进一步探讨。
OBJECTIVE: To understand the drug resistance of VP and to find the prevailing predominance and to provide a basis for retrospecting pathogens and treatment. Methods: Separation of seafood by VP was carried out by screening method, GB method was used in patients, serotyping by diagnostic serum and genotyping by PFGF, and drug resistance by K-B method. Results: 1040 strains of VP were divided into 10 serotypes, with a genotyping rate of 94.64%. The prevalence of VP in seafood was 0: 6 and 0: 5, and VP was 0: 3. VP was more sensitive to ampicillin The drug rate was high, reaching 92.64%, no multi-resistant bacteria were detected. According to PFGE change, VP was divided into 12 types, seafood VP was 29 type, and type 1 was prevalent type. Conclusion: The serotypes and PFGE types of seafood VP in Ningbo are more dispersed than those in patients with VP. On the contrary, there is a significant difference between the prevailing strains and the epidemic predominant strains, which indicates that we can trace and trace the serotypes and tracing strains with VPGE. High resistance, is widely used with the need to further explore.