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目的 建立肾上腺髓质素 (ADM)放射免疫分析法 (RIA) ,探讨人及动物心血管生理病理时ADM的变化。方法 制备特异性抗ADM血清 ,用氯胺 T法制备1 2 5I ADM ,建立了灵敏的ADMRIA ,对正常人、冠心病、高血压患者进行血浆ADM检测 ,同时观察1 2 5I ADM于大鼠尾静脉注射 6h后在各脏器中的分布 ,测定鼠血管内皮细胞和平滑肌细胞 12 ,2 4,36 ,48和 72h细胞培养上清液的ADM含量。结果 该方法的灵敏度为 2 32ng L。高血压、冠心病患者ADM明显高于对照组。1 2 5I ADM在鼠各脏器的分布以肾脏最高 ,肺、血管内皮次之 ,平滑肌最低。鼠血管内皮细胞和平滑肌细胞上清细胞培养ADM含量峰值在 2 4h ,分别为 46 5 4ng L和 6 71ng L。结论 ADMRIA可为心血管疾病的研究、诊断和治疗提供依据。
Objective To establish a radioimmunoassay (RIA) method of adrenomedullin (ADM) to investigate the changes of ADM in the cardiovascular and pathophysiology of human and animal. Methods Specific anti-ADM serum was prepared, and 125I ADM was prepared by chloramine T method. A sensitive ADMRIA was established to detect plasma ADM in normal subjects, patients with coronary heart disease and hypertension. Meanwhile, The content of ADM in the culture supernatant of rat vascular endothelial cells and smooth muscle cells at 12, 24, 36, 48 and 72 hours after intravenous injection of 6h were determined. Results The sensitivity of this method was 2 32 ng L. ADM in patients with hypertension and coronary heart disease was significantly higher than that in the control group. 1 2 5I ADM in the distribution of rats in the highest kidney, lung, vascular endothelium, the lowest smooth muscle. The peak of ADM content in rat vascular endothelial cell and smooth muscle cell supernatant was cultured for 24 h, 465 4 ng L and 6 71 ng L, respectively. Conclusion ADMRIA can provide the basis for the research, diagnosis and treatment of cardiovascular diseases.