通过试验评价了作为三次采油技术的MEOR方法,该方法利用微生物活动提高伊朗油藏采收率,在试验过程中特别关注了产生生物表面活性剂在MEOR动态中的作用。通过把Taguchi方法的正交阵列作为试验设计技术设计并且进行了一些试验,以便探索和评价微生物配方对采收率的影响和在采油过程中产生生物表面活性剂的重要性。以两种主要模式进行了所有的试验,即烧瓶模式和简单岩心驱替系统模式。研究了5个参数的作用及其重要性,这5个参数是碳源、氮和磷量、微生物类型、温度和盐浓度,一个参数分为3个级。最终结果显示,与水驱采油(不进行微生物处理)相比,在开采过程中进行微生物处理提高了采收率,而且最终结果还证明,在微生物采油的不同机理中,产生生物表面活性剂在本项特殊研究中起重要作用。当在45℃的条件下把Payrdar油田的本源微生物(这种微生物是从原油样品中分离出来的)与2%蔗糖(作为主要碳源)和0.2%(NH4)2HPO4-起使用时,这些不同试验中的最高采收率约为14.3%。当在1%的盐浓度中把枯草芽孢杆菌(B.Subtilis)与2%蔗糖和0.4%(NH4)2HPO4一起使用时,观测到了产生生物表面活性剂的最佳结果。 MEOR, a tertiary oil recovery technology, was evaluated through experiments that utilized microbial activity to increase oil recovery in Iran. Particular attention was paid to the role of biogenic surfactants in the MEOR dynamics during the test. An orthogonal array of Taguchi methods was designed and tested as an experimental design technique to explore and evaluate the impact of microbial formulations on oil recovery and the importance of producing biosurfactants during oil recovery. All the tests were done in two main modes, the flask mode and simple core-displacement system mode. The role and importance of five parameters were studied. The five parameters were carbon source, nitrogen and phosphorus content, microbial type, temperature and salt concentration. One parameter was divided into three levels. The end result showed that microbial recovery during recovery was enhanced compared to waterflood (without microbial treatment), and the end result also demonstrated that in different mechanisms of microbial recovery biosurfactants are produced at This special study plays an important role. These differences were observed when the native microbes of the Payrdar field, which were isolated from the crude oil samples, were used at 45 ° C with 2% sucrose (as the main carbon source) and 0.2% (NH4) 2HPO4 The maximum recovery in the test was about 14.3%. The best results for biosurfactants were observed when B. subtilis was used with 2% sucrose and 0.4% (NH4) 2HPO4 at 1% salt concentration.