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目的:建立采用超声波提取,高效液相色谱法测定新疆阔克阿木提果皮中绿原酸和芦丁的含量的方法。方法:色谱柱:安捷伦HC-C18柱(250mm×4.6mm,5μm);流动相体积比50∶50的甲醇-0.4%磷酸溶液;检测波长为360nm;柱温为30℃;流速为1.0ml/min低压梯度洗脱。结果:绿原酸和芦丁分别在12.5~150μg/ml(r=0.9998)和12.5~150μg/ml(r=0.9998)浓度范围内呈良好的线性关系,平均回收率分别为101.08%(RSD=1.38%,n=5)和99.56%(RSD=1.17%,n=5)。结论:该方法简便快速、重现性好、准确可靠,适用于新疆阔克阿木提果皮中绿原酸和芦丁的含量测定。
OBJECTIVE: To establish a method for the determination of chlorogenic acid and rutin in the peel of Amomum villosum by ultrasonic extraction and high performance liquid chromatography. Method: Column: Agilent HC-C18 column (250mm × 4.6mm, 5μm); methanol-0.4% phosphoric acid solution with a mobile phase volume ratio of 50:50; detection wavelength of 360nm; column temperature of 30 ℃; min low pressure gradient elution. RESULTS: Chlorogenic acid and rutin had a good linear relationship in the range of 12.5-150 μg / ml (r = 0.9998) and 12.5-150 μg / ml (r = 0.9998), with the average recoveries of 101.08% (RSD = 1.38%, n = 5) and 99.56% (RSD = 1.17%, n = 5). Conclusion: The method is simple and rapid, reproducible, accurate and reliable, and is suitable for the determination of chlorogenic acid and rutin in the peel of Amurum.