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为了探讨蛛网膜下腔出血（SAH）后蛛网膜下腔红细胞溶解过程中自由基代谢的变化，以及在迟发性脑血管痉挛（DCVS）发病机理中的作用，我们采用Wistar鼠的新鲜血制备成新鲜红细胞（FRBC）与孵育红细胞（IRBC），对FRBC与IRBC中氧自由基（OFRS）含量、超氧化物岐化酶（Cu-ZnSOD）活性、脂质过氧化物（LPO）和蛋白质碳基（Cb）衍生物含量进行了测定。结果表明：IRBC与FRBC比较，IRBC中OFRs含量显著升高（P＜0.05），Cu-ZnSOD活性显著降低（P＜0.01），LPO含量显著升高（P＜0.01），Cb含量显著升高（P＜0.01）。提示红细胞在孵育溶解的过程中有自由基代谢的紊乱。我们讨论了自由基介导的病理损伤在DCVS病理中的作用。
To investigate the changes of free radical metabolism during subarachnoid erythrocyte lysis after subarachnoid hemorrhage (SAH) and its role in the pathogenesis of delayed cerebral vasospasm (DCVS), we used fresh blood from Wistar rats (FRBC) and incubated erythrocytes (IRBC) on the contents of oxygen free radical (OFRS), superoxide dismutase (Cu-ZnSOD), lipid peroxides (LPO) and protein carbon The content of Cb derivatives was determined. The results showed that the content of OFRs in IRBC was significantly increased (P <0.05), the activity of Cu-ZnSOD was significantly decreased (P <0.01), the content of LPO was significantly increased (P <0.01) and the content of Cb was significantly increased P <0.01). Prompt red blood cells in the incubation process of dissolution of free radical metabolism. We discuss the role of free radical-mediated pathological damage in DCVS pathology.