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在云南本地芥菜型油菜文山大脚黄(母本)与甘蓝型油菜品种花油5号(父本)杂交F1小孢子培养获得的DH群体中分离出双主茎材料,其田间表现具有良好的抗旱性。利用cDNA-AFLP技术分析七叶期甘蓝型油菜双主茎材料在干旱和对照条件下基因差异表达情况。共筛选了512对引物组合,获得56个干旱诱导上调表达的差异片段。对36个大于100 bp的片段克隆测序、同源比对、结果归并为30个非重复的TDFs,对已知功能的18个TDFs功能预测表明这些片段参与了细胞形态建成与降解、代谢、能量、细胞周期、蛋白质合成、蛋白质修饰、蛋白质结合、运输途径等相关过程。本试验获得的差异表达基因对于了解油菜苗期抗旱性形成的调控机制具有一定的参考价值。
The double main stem material was isolated from the DH population obtained from the F1 microspore culture of F1 hybrids of Mustard (Balsam) Drought resistance. Using cDNA-AFLP technique to analyze the differential gene expression between the two main stem materials of Brassica napus in drought and control conditions. A total of 512 pairs of primers were screened and 56 drought-induced up-regulated differentially expressed fragments were obtained. Thirty-six TDFs were cloned and sequenced in 36 fragments greater than 100 bp, and the results were grouped into 30 non-repetitive TDFs. The prediction of the function of 18 TDFs with known functions indicated that these fragments are involved in cell morphogenesis and degradation, metabolism, energy , Cell cycle, protein synthesis, protein modification, protein binding, transport and other related processes. The differentially expressed genes obtained in this experiment have some reference value for understanding the regulation mechanism of drought resistance in rape seedlings.