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目的比较不同来源及不同批次尿细胞诱导多功能干细胞的效率。方法收集夜尿样本25份及非夜尿样本81份(其中31份来自男性,50份来自女性),采用非整合、无血清、无饲养层细胞、无致癌基因c-MYC的重编程法诱导获得诱导多能干细胞(induced pluripotent stem cells,i PSCs)。经碱性磷酸酶(alkaline phosphatase,AP)染色估算尿细胞重编程效率。结果夜尿与非夜尿中尿细胞分离的成功率分别为8%和44%,差异有统计学意义(P<0.05);同一来源不同批次尿细胞间及不同尿液来源尿细胞间的重编程效率的差异均有统计学意义(P<0.05)。结论夜尿不适宜作为收集尿细胞的来源。不同的尿细胞会影响重编程的效率,本实验为提高诱导效率的研究奠定了基础。
Objective To compare the efficiency of induced pluripotent stem cells from different sources and batches of urine cells. Methods Totally 25 samples of nocturia and 81 samples of non-nocturia (31 males and 50 females) were collected, and non-integrated, serum-free and feeder-free cells were used to induce the reprogramming of the oncogene c-MYC Induced pluripotent stem cells (iPSCs) were obtained. Urine cell reprogramming efficiency was estimated by alkaline phosphatase (AP) staining. Results The success rates of nocturnal and non-nocturia urinary cells were 8% and 44%, respectively, with significant difference (P <0.05). Urine and intercellular urine Reprogramming efficiency differences were statistically significant (P <0.05). Conclusion Nocturia is not suitable as a source of urine cells. Different urine cells affect the efficiency of reprogramming, this experiment lays the foundation for the study of improving the induction efficiency.