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将羊外周血淋巴细胞ｍＲＮＡ注入非洲爪蟾卵母细胞胞浆内，借助卵母细胞的翻译系统，表达羊外周血淋巴细胞的Ｍ型乙酰胆碱（Ａｃｈ）受体并移植到卵母细胞膜上，用电压钳位仪检测表达受体电流。结果发现Ａｃｈ可诱发卵母细胞产生电流反应，该反应能被Ｍ型Ａｃｈ受体阻断剂硫酸阿托品阻断，但不能被Ｎ型受体阻断剂氯化筒箭毒阻断，提示：淋巴细胞存在Ｍ型Ａｃｈ受体，其基本特性为：１）Ａｃｈ浓度为１０－８～１０－３ｍｏｌ／Ｌ时，与电流幅度呈线性关系，１０－６ｍｏｌ／ＬＡｃｈ为１／２最大有效浓度，产生的电流幅度为３８００±１１１６ｎＡ；２）当钳制电位在－８０～±２０ｍＶ范围内，ＶＩ关系曲线为直线，逆转电位约为－２０±１２５ｍＶ，该值接近卵母细胞的Ｃｌ－平衡电位；３）用ＳＯ２－４取代灌注液中５０％的Ｃｌ－，电流幅度下降４４８９％，逆转电位正向偏移１６±１ｍＶ。以上结果进一步证明Ｃｌ－是表达Ｍ型Ａｃｈ电流的主要载流离子。淋巴细胞表达Ｍ型受体的Ａｃｈ电流证明羊淋巴细胞上有完整的Ｍ型Ａｃｈ受体。
The sheep peripheral blood lymphocyte mRNA was injected into the cytoplasm of Xenopus laevis oocytes, the Mch acetylcholine (Ach) receptor of goat peripheral blood lymphocytes was expressed and transplanted onto the oocyte membrane by the oocyte translation system, Voltage clamp assays detect receptor current. The results showed that Ach induced the generation of current response in oocytes, which could be blocked by the M-type Ach receptor antagonist atropine sulfate but not blocked by the N-type blocker, There are M type Ach receptors in cells, the basic characteristics are as follows: 1) Ach concentration of 10-8 ~ 10-3mol / L, and the current amplitude of a linear relationship, 10-6mol / LAch 1/2 maximum effective concentration, resulting Of the current amplitude 38 00 ± 11 16nA; 2) When the clamp potential in the range of -80 ~ ± 20mV, V I relationship curve is straight line, reversal potential of about -20 ± 1 25mV, the value close to the oocyte Cl-equilibrium potential of cells; 3) Substituting SO2-4 for 50% of Cl- in perfusate, the current amplitude decreased by 4489% and the reverse potential shifted by 16 ± 1mV. The above results further prove that Cl- is the main carrier ion that expresses M type Ach current. Ach currents of lymphocyte-expressing M-type receptors demonstrated complete M-type Ach receptors on goat lymphocytes.