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我们在建立了检测HAAg、抗HAV 和抗HAV-IgM 的ELISA 方法、并确定了各自的特异性之后,对实际标本进行了检测,其中双夹心法还作了与进口试剂的对比研究。结果表明:检测HAAg 可使约50%的甲肝病人得到早期诊断;在50～60%甲肝病人的双份血清中可见有抗HAV 滴度≥10倍增长;检测抗HAV-IgM 是目前较理想的甲型病毒性肝炎的早期诊断方法。
After we established ELISA methods for detecting HAAg, anti-HAV and anti-HAV-IgM and determined their own specificities, we tested the actual samples. The double-sandwich method was also used to compare with imported reagents. The results showed that the detection of HAAg can make early diagnosis of about 50% of patients with hepatitis A; the anti-HAV titer of ≥10 times increase can be seen in 50-60% of hepatitis A patients; the detection of anti-HAV-IgM is more ideal Early diagnosis of hepatitis A virus.