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目的 探讨我国北方汉族人矽肺的易感性与人类白细胞抗原 (HLA) DRB1 、DQB1 位点等位基因多态的相关性。方法 用序列特异性引物的聚合酶链式反应 (PCR SSP)方法 ,分析 48名汉族矽肺患者及 10 0名汉族无血缘关系对照者 (均为有过 14年以上接尘史的井下凿岩工 )HLA DRB1 、DQB1 位点的基因频率分布 ,以相对危险度 (RR)代表相关程度。结果 在矽肺患者组中 ,DRB1 140 1、DQB1 0 5的等位基因频率明显高于对照组 ,经统计学处理 ,两组间差异具有显著性 (χ2 =5 .6 1,P =0 .0 0 6 6 ,RR =17.40 ;χ2 =10 .70 ,P =0 .0 0 11,RR =3.81) ;而DRB1 0 9的等位基因频率则明显低于对照组 ,差异有显著性 (χ2 =5 .70 ,P =0 .0 187,RR =0 .2 1) ;其他等位基因频率的分布差异无显著性 (P >0 .0 5 )。结论 HLA DRB1 140 1、DQB1 0 5可能与矽肺的易感性有关 ,而DRB1 0 9则可能与机体抗矽肺的保护性有关。HLA DR位点与机体易感及保护的双重作用有关 ,等位基因之间的共同作用可能是影响矽肺发生的原因之一。
Objective To investigate the association between the susceptibility of silicosis in Han population in northern China and the alleles of DRB1 and DQB1 loci in human leukocyte antigen (HLA). Methods PCR-SSP method was used to analyze 48 Han silicosis patients and 100 Han unrelated control subjects (all of them were downhole rock drillers who had been exposed to dust for more than 14 years ) HLA DRB1, DQB1 locus gene frequency distribution, the relative risk (RR) represents the degree of correlation. Results The frequencies of alleles of DRB1 140 1 and DQB1 05 in patients with silicosis were significantly higher than those in controls, and the difference was statistically significant (χ2 = 5.16, P = 0.0) The frequency of allele in DRB1 0 9 was significantly lower than that in control group (χ2 = 0.66, RR = 17.40; χ2 = 10 .70, P = 0.0001, RR = 3.81) 5.70, P = 0.087, RR = 0.21). There was no significant difference in the distribution of other alleles (P> 0.05). Conclusion HLA DRB1 140 1 and DQB1 05 may be related to the susceptibility of silicosis, while DRB1 0 9 may be related to the protective effect of anti-silicosis on the body. HLA DR locus and the body’s dual role of susceptibility and protection, allele synergy between the impact of silicosis may be one of the reasons.