Bioactivity of seagrass against the dengue fever mosquito Aedes aegypti larvae

来源 :Asian Pacific Journal of Tropical Biomedicine | 被引量 : 0次 | 上传用户:hwhxl0
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Objective:To identify the larvieidal activity of the seagrass extracts.Methods:Seagrass extracts,Syringodium isoelifolium(.S.isoetifolium),Cymodocea serrulata and Hulophila beccarii,were dissolved in DMSO to prepare a graded series of concentration.Batches of 25 early 4th instars larvae of Aedes aegypti(Ae.aegypti) were transferred to 250 ml.enamel bowl containing199 mL of distilled water and 1 mL of plant extracts(0.01 mg- 0.1 mg).After 24 h the mortality rate was identified with the formulae[(%of lest mortality-%of control mortality)/(100-%of control morlality)]× 100.Each experiment was conducted with three replicates and a concurrent control group.A control group consisted of 1 mL of DMSO and 199 mL of distilled water only.Results:The root extract of S.isoelifolium showed maximum larvicidal activity with minimum concentration of extract of LC_(50)= 0.0604 ± 0.0040)μg/mL with lower confidence limit(LCL)-upper confidence limit(UCL) =(0.051-0.071) and LC_(90)=0.0972 μg/mL followed by leaf extract of S.isoelifolium showed LC_(50)=(0.062 ± 0.005)μ’g/mL.The regression equation of pool and leaf extract of S.isoelifolium for4th instar larvae were Y =4.909- 1.32x(R~2= 0.909) and Y= 2.066+ 1.2x(R~2 =0.897) respectively.The results of the preliminary photochemical constituents shows the presence of saponin,steroids,terpenoid,phenols,protein and sugars.Conclusions:from the present study the ethanolic extracts of seagrass of S.isoelifolium possesses lead compound for development of larvieidal activity. Objective: To identify the larvieidal activity of the seagrass extracts. Methods: Seagrass extracts, Syringodium isoelifolium (.S.isoetifolium), Cymodocea serrulata and Hulophila beccarii, were dissolved in DMSO to prepare a graded series of concentrations. Batches of 25 early 4th instars larvae of Aedes aegypti (Ae. aegypti) were transferred to 250 ml. enamel bowl containing 199 mL of distilled water and 1 mL of plant extracts (0.01 mg- 0.1 mg). After 24 h the mortality rate was identified with the formula [ of lest mortality-% of control mortality / (100-% of control morlality)] × 100.Each experiment was conducted with three replicates and a concurrent control group. A control group consisted of 1 mL of DMSO and 199 mL of distilled water only.Results: The root extract of S.isoelifolium showed maximum larvicidal activity with minimum concentration of extract of LC 50 = 0.0604 ± 0.0040) μg / mL with lower confidence limit (LCL) -upper confidence limit (UCL) = (0.051 -0.071) and LC_ (90) = 0.0972 μg / mL followed by leaf extract of S. isoelifolium showed LC_ (50) = (0.062 ± 0.005) μ’g / mL.The regression equation of pool and leaf extract of S.isoelifolium for4th instar larvae were Y = 4.909-1.32x (R ~ 2 = 0.909) and Y = 2.066 + 1.2x (R ~ 2 = 0.897) respectively.The results of the preliminary photochemical constituents shows the presence of saponin, steroids, terpenoid, phenols, protein and sugars. Conclusions: from the present study of the ethanolic extracts of seagrass of S.isoelifolium possesses lead compound for development of larvieidal activity.
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