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目的检测miR-21在甲状腺乳头状癌(PTC)患者肿瘤组织和血清中的表达情况,探讨其临床意义。方法采用实时荧光定量聚合酶链反应(Real-time RT-PCR)检测46例PTC患者肿瘤组织和血清中miR-21表达情况,并与35例结节性甲状腺肿患者和18例正常人进行对比分析。结果 PTC患者肿瘤组织和血清miR-21表达相对水平为2.74±1.53和0.21±0.08,明显高于结节性甲状腺肿患者(0.92±0.81和0.08±0.05)和正常人(0.86±0.39和0.07±0.03)(P<0.05)。PTC患者肿瘤组织和血清中miR-21表达具有一致性。PTC患者肿瘤组织和血清中miR-21的表达与腺外侵润、远处转移、淋巴结转移和不同TNM分期有关(P<0.05)。结论 miR-21在PTC患者肿瘤组织和血清中的表达明显升高,且其表达水平与PTC临床病理特征相关,可能是新的PTC早期检测指标。临床上对于PTC肿瘤组织和血清中miR-21的检测可能用于PTC早期临床诊断。
Objective To detect the expression of miR-21 in the tumor tissues and serum of patients with papillary thyroid carcinoma (PTC) and to investigate its clinical significance. Methods Real-time RT-PCR was used to detect the expression of miR-21 in tumor tissue and serum of 46 patients with PTC and compared with 35 patients with nodular goiter and 18 healthy controls analysis. Results The relative levels of miR-21 in tumor tissue and serum of patients with PTC were 2.74 ± 1.53 and 0.21 ± 0.08, significantly higher than those of nodular goiter (0.92 ± 0.81 and 0.08 ± 0.05) and normal subjects (0.86 ± 0.39 and 0.07 ± 0.03) (P <0.05). The expression of miR-21 in tumor tissues and serum of PTC patients is consistent. The expression of miR-21 in tumor tissue and serum of patients with PTC was related to infiltration of extra-gland, distant metastasis, lymph node metastasis and TNM stage (P <0.05). Conclusion The expression of miR-21 in tumor tissue and serum of patients with PTC is significantly increased, and its expression level is correlated with clinicopathological features of PTC, which may be a new early detection index of PTC. The clinical detection of miR-21 in PTC tumor tissues and serum may be used for early clinical diagnosis of PTC.