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为了对转1Dx5基因小麦的T1进行遗传分析,将优质高分子量麦谷蛋白亚基(HMW-GS)基因1Dx5转入新疆耐盐小麦品种新冬26,在蛋白质水平上经SDS-PAGE检测,有37粒小麦种子1Dx5基因表达。经过继代培育,得到37个T1转基因株系,SDS-PAGE分析T1转基因小麦籽粒的HMW-GS组成,结果表明:在蛋白质水平,HMW-GS的组成在T1小麦种子中有3种表型:表型Ⅰ,外源1Dx5基因没有表达;表型Ⅱ,外源1Dx5基因表达;表型Ⅲ,内源1Dx2基因没有表达。这3种表型出现的概率分别约为34.3%,45.9%和19.8%。该研究成功获得了新的HMW-GS组成的小麦,为选育优质小麦提供分子依据。
In order to analyze the genetic diversity of T1 transgenic wheat 1Dx5, the 1Dx5 high-quality HMW glutenin subunit (HMW-GS) gene was transferred into Xinjiang Xinmai26, a salt tolerant wheat cultivar Xinjiang. SDS- Wheat seed 1Dx5 gene expression. After T1 generation, 37 T1 transgenic lines were obtained. The HMW-GS composition of T1 transgenic wheat grain was analyzed by SDS-PAGE. The results showed that there are three phenotypes of HMW-GS in T1 wheat seed at the protein level: Phenotype Ⅰ, exogenous 1Dx5 gene is not expressed; phenotype Ⅱ, exogenous 1Dx5 gene expression; phenotype Ⅲ, endogenous 1Dx2 gene is not expressed. The probabilities of these three phenotypes were about 34.3%, 45.9% and 19.8% respectively. The study successfully obtained a new HMW-GS composition of wheat, providing molecular basis for breeding high-quality wheat.