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21-三体综合征是染色体异常导致的疾病,通过重编程21-三体综合征患儿两种组织来源的细胞成为多能干细胞,比较两种组织来源的细胞建立21-三体综合征诱导多能干细胞(T21-iPSCs)系的效率,为进一步研究21-三体综合征发病机制提供细胞模型,并为选择高效制备T21-iPSCs的组织类型提供理论依据。该实验利用慢病毒介导4种转录因子(Oct4、Sox2、Klf4、c-Myc)分别诱导人21-三体综合征的羊水细胞和胎儿皮肤成纤维细胞,建立诱导多能干细胞系(Trisomy 21 human amniotic fl uid induced pluripotent stem cells,T21 hAF-iPSCs;Trisomy 21 human dermal fi broblast induced pluripotent stem cells,T21 hDF-iPSCs),T21 hAF-iPSCs及T21 hDF-iPSCs在蛋白和mRNA水平上均表达人胚胎干细胞的多能性分子标记,如Oct4、Nanog等,具有在体外及体内分化三个胚层的能力,其在培养过程中能维持异常核型并能维持自我更新状态。结果发现,利用羊水细胞建立T21-iPSCs效率高于皮肤成纤维细胞,羊水细胞可能是制备T21-iPSCs的理想细胞类型。
21-trisomy syndrome is a disease caused by chromosomal abnormalities, and pluripotent stem cells are reprogrammed by reprogramming the cells derived from both tissues in children with trisomy 21, and comparing the two tissue-derived cells to establish 21-trisomy syndrome induction (T21-iPSCs) lines, provide a cellular model for further studying the pathogenesis of 21-trisomy syndrome and provide a theoretical basis for selecting tissue types for efficient preparation of T21-iPSCs. In this experiment, we used lentivirus-mediated four transcription factors (Oct4, Sox2, Klf4, c-Myc) to induce amniotic fluid cells and fetal skin fibroblasts in human trisomy 21 respectively to establish induced pluripotent stem cell line (Trisomy 21 human amniotic fl uid induced pluripotent stem cells (T21 hAF-iPSCs); human embryonic fibroblasts (T21 hDF-iPSCs), T21 hAF-iPSCs and T21 hDF-iPSCs Pluripotent molecular markers of stem cells, such as Oct4, Nanog, etc., have the ability to differentiate three germ layers in vitro and in vivo, maintain abnormal karyotypes and maintain self-renewal during culture. The results showed that the use of amniotic fluid cells to establish T21-iPSCs is more efficient than skin fibroblasts, amniotic fluid cells may be the ideal cell preparation T21-iPSCs.