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在生物标本制备中,一般都强调固定的温度必须在4°—0℃之间。由于温度低,可以减少细胞物质的抽提。因此,目前不论何种生物标本大多数都是低温下固定。由于温度降低,固定剂渗透的速度和深度以及固定剂与细胞物质的相互作用也相应的降低了,为了克服这个缺点,必须延长固定的时间。固定的温度能否升高呢?事实上,只要时间控制在一定的范围内,在常温下细胞物质的抽提并不严重影响细胞的超微结构。相反可以改善细胞的固定质量,提高细胞结构的清晰度和对比度。我们用小白鼠肝、肾和肠等组织分成两组:甲组用磷酸缓冲的5 ％戊二醛和1％O、O_4在0—4℃下双固定,
In the preparation of biological specimens, it is generally emphasized that the fixed temperature must be between 4 ° to 0 ° C. Due to low temperature, can reduce the extraction of cellular material. Therefore, at present, most of the biological specimens are fixed at low temperature. As the temperature decreases, the rate and depth of penetration of the fixative and the interaction of the fixative with the cellular material are correspondingly reduced, and in order to overcome this disadvantage, it is necessary to prolong the fixed time. In fact, as long as the time control in a certain range, at room temperature, the extraction of cellular material does not seriously affect the cell ultrastructure. On the contrary can improve the fixed quality of cells, improve the clarity and contrast of the cell structure. We used mice liver, kidney and intestine tissue divided into two groups: A group with phosphate buffered 5% glutaraldehyde and 1% O, O_4 at 0-4 ° C double fixed,