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目的:观察活血降脂保肝汤(HJBD)对非酒精性脂肪性肝病(NAFLD)大鼠肝组织微粒体甘油三脂转运蛋白(MTP)mRNA及蛋白表达的影响,探讨其防治NAFLD可能的作用机制。方法:取健康雄性SD大鼠60只随机分为6组,正常组,模型组,HJBD低、中、高剂量组(6.48,13.68,27.36 g·kg-1)及强肝片组(0.067 g·kg-1)。除正常组外,其余各组给予高脂饮食8周后,用低、中、高剂量HJBD及强肝片进行干预,连续ig 10周。采集空腹血分离血清检测各组大鼠血脂情况,取肝组织行苏木素-伊红(HE)观察大鼠肝组织病理变化,逆转录-聚合酶链式反应(RT-PCR)检测MTP mRNA及蛋白的表达情况。结果:免疫组化及RT-PCR检测结果显示,与正常组比较,模型组MTP表达水平降低,差异有统计学意义(P<0.01);与模型组比较,HJBD各剂量组及强肝片组MTP表达均升高,差异有统计学意义(P<0.01,P<0.05),以高剂量组升高最明显;血脂紊乱及肝脂肪变性也较模型组有不同程度改善。结论:HJBD对高脂饮食诱导的NAFLD大鼠有较好的治疗作用,推测MTP可能是HJBD抗NAFLD的有效作用靶点。
Objective: To observe the effect of HJBD on liver microsomal triglyceride transport protein (MTP) mRNA and protein expression in nonalcoholic fatty liver disease (NAFLD) rats and to explore the possible role of HJBD in preventing and treating NAFLD mechanism. Methods: Sixty healthy male Sprague-Dawley rats were randomly divided into 6 groups: normal group, model group, HJBD low, medium and high dose groups (6.48, 13.68, 27.36 g · kg-1) Kg-1). In addition to the normal group, the remaining groups were given high-fat diet for 8 weeks, with low, medium and high doses of HJBD and Qianggan intervention, continuous ig 10 weeks. Blood samples were collected from fasting blood to determine the serum lipids in rats. Pathological changes of liver were observed by hematoxylin-eosin (HE) and MTP mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) The expression of the situation. Results: The results of immunohistochemistry and RT-PCR showed that compared with the normal group, the expression level of MTP in the model group decreased significantly (P <0.01), compared with the model group, HJBD dose groups and Qianggan Tablets MTP expression increased, the difference was statistically significant (P <0.01, P <0.05), the most obvious increase in high-dose group; dyslipidemia and hepatic steatosis than the model group to varying degrees improve. Conclusion: HJBD has a good therapeutic effect on NAFLD rats induced by high-fat diet. It is speculated that MTP may be an effective target of HJBD against NAFLD.