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十字花科许多植物表现为异花授粉和较高的种间杂交亲和性,这些生殖特征喻示着外源基因在该科植物间存在潜在的流动风险.通过苯胺蓝荧光法观察、人工杂交、混合花粉蒙导授粉结合杂交后代的分子鉴定,研究了甘蓝型抗草甘膦油菜Q3与5种十字花科植物(共6个材料)的杂交亲和性,以评估外源抗除草剂基因在上述植物间的流动.结果表明,甘蓝型油菜2个品种、雪里蕻、小白菜与Q3杂交的亲和指数分别为16.15,12.77,2.345和0.85,F1代植株PCR检测为阳性;诸葛菜、播娘蒿×Q3表现出高度不亲和,荧光显微镜观察发现花粉管生长受阻,分别停滞在诸葛菜花柱上部1/3处与播娘蒿柱头表面,蒙导后代植株PCR检测均表现阴性.在大田邻近间隔种植和自然授粉条件下,Q3与甘蓝型油菜2个品种、雪里蕻、小白菜间的基因流频率分别为2.3278%,2.1487%,1.0157%和0.9236%,与诸葛菜、播娘蒿2种杂草的频率为0.基因流频率与杂交亲和性高度相关.本研究结果同时说明十字花科植物间的外源基因流动不容忽视.
Many cruciferous plants showed cross-pollination and higher interspecific hybridization affinity, these reproductive traits show that exogenous genes in the families of plants there is a potential flow risk.Aniline blue fluorescence observation, artificial hybridization , Mixed pollen Mongolian pollination and hybrid progeny were studied. The compatibility of Q3 with glyphosate resistant Brassica napus and five species of cruciferous plants (6 materials in total) was studied in order to evaluate the effect of exogenous herbicide resistant gene Flowering among the above plants.The results showed that the affinity index of two varieties of Brassica napus, X. rubrum and Pakchoi were 16.15, 12.77, 2.345 and 0.85, respectively, and the F1 plants were positive by PCR; Artemisia × Q3 showed a high degree of incompatibility, fluorescence microscopy showed obstruction of pollen tube growth, respectively, stagnant in the upper part of Zhuge Caragana stigma surface and stems, Meng Gui offspring plant PCR tests were negative in the field The results showed that the frequency of gene flow between two cultivars of Q3 and B. napus was 2.3278%, 2.1487%, 1.0157% and 0.9236%, respectively. The frequency of gene flow between Q3 and Brassica napus was significantly higher Weed frequency 0. frequency of gene flow hybridization affinity are highly correlated. The results of this study also indicated that foreign gene flow among plants can not be ignored.