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目的探讨芪药鸡金粥对化疗大鼠胃肠黏膜机械屏障损伤的保护作用,从ERK/MAPK通路的角度探讨机制。方法将48只SPF级Wistar大鼠随机分为药膳组(药膳灌胃)、模型组(白粥灌胃)、空白组(白粥灌胃)各16只,于实验第7天,药膳组和模型组行氟尿嘧啶150mg/kg一次性腹腔注射,制备胃肠黏膜损伤大鼠模型。化疗后第1天和第8天,每组各随机抽取8只大鼠处死后取回肠组织,采用蛋白质印迹法检测相关指标。结果化疗后第1天,模型组连接蛋白显著下降(均P<0.05),空白组各通路蛋白的表达水平显著或相对低于同期药膳组和模型组水平(均P<0.05);化疗后第8天,药膳组Claudin-1连接蛋白表达显著高于模型组(P<0.05),各通路蛋白表达显著高于同期空白组(均P<0.05),模型组ERK1/2蛋白表达显著高于空白组(P<0.05)。结论芪药鸡金粥可调节大鼠肠道机械屏障紧密连接蛋白的表达,从而保护机械屏障的结构与功能,可能是通过ERK/MAPK通路的活化而发挥保护作用。
Objective To investigate the protective effect of Qiluji Ginkgo poria on the damage of gastrointestinal mucosal mechanical barrier in rats and explore the mechanism from the perspective of ERK / MAPK pathway. Methods Forty-eight SPF Wistar rats were randomly divided into four groups: medicated group (fed with medicated gavage), model group (fed with porridge), and blank group (fed with porridge). On the 7th day, Fluorouracil 150mg / kg intraperitoneal injection, preparation of rat model of gastrointestinal mucosal injury. On the 1st day and the 8th day after chemotherapy, 8 rats in each group were sacrificed and the intestine was removed after sacrifice. Western blotting was used to detect the relevant indexes. Results On the first day after chemotherapy, the expression of connexin in the model group was significantly decreased (all P <0.05), and the protein expression of each pathway in the blank group was significantly or relatively lower than that in the same diet group and model group (all P <0.05) On day 8, the expression of Claudin-1 in the diet group was significantly higher than that in the model group (P <0.05), and the protein expression of each pathway was significantly higher than that in the blank control group (all P <0.05). The ERK1 / 2 protein expression in the model group was significantly higher than that in the blank group Group (P <0.05). CONCLUSIONS: Astragalus membranaceus porridge can regulate the expression of tight junction protein (APP) in the mechanical barrier of intestinal tract in rats and thus protect the structure and function of mechanical barrier. It may play a protective role through the activation of ERK / MAPK pathway.