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目的研究CD23/sCD23、CD19在慢性肾功能衰竭(CRF)发病机制中的作用及CD23、CD19之间的相互关系。方法CRF患者25例及健康体检者20人,分别抽取非抗凝、肝素抗凝静脉血各2ml,采用流式细胞仪分析检测外周血CD23+、CD19+及CD23+CD19+淋巴细胞百分率,用ELISA法检测sCD23水平。结果CRF组外周血CD23+淋巴细胞百分率[(4.19±0.93)%]及CD23+CD19+淋巴细胞百分率[(3.21±1.30)%]显著低于正常对照组[(5.33±1.74)%,(4.79±2.18)%],差异有高度统计学意义(P<0.01)。血清sCD23水平CRF组[(173.2±60.3)U/ml]显著高于正常对照组[(50.1±7.6)U/ml],差异有高度统计学意义(P<0.01)。结论CD23是引起CRF患者免疫紊乱的重要因素。
Objective To investigate the role of CD23 / sCD23 and CD19 in the pathogenesis of chronic renal failure (CRF) and the relationship between CD23 and CD19. Methods Twenty-five CRF patients and 20 healthy volunteers were enrolled in this study. Blood samples of non-anticoagulant and heparin-anticoagulated venous blood were collected respectively. The percentages of CD23 +, CD19 + and CD23 + CD19 + lymphocytes in peripheral blood were detected by flow cytometry (FCM) sCD23 level. Results The percentage of CD23 + lymphocytes (4.19 ± 0.93)% and CD23 + CD19 + lymphocytes (3.21 ± 1.30%) in CRF group were significantly lower than those in control group [(5.33 ± 1.74)% vs (4.79 ± 2.18 )%], The difference was highly statistically significant (P <0.01). The level of serum sCD23 in CRF group [(173.2 ± 60.3) U / ml] was significantly higher than that in control group [(50.1 ± 7.6) U / ml], the difference was statistically significant (P <0.01). Conclusion CD23 is an important factor in causing immune disorder in CRF patients.