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目的:建立乌参八味胶囊的质量标准。方法:采用薄层色谱法,对该品种中的何首乌、川芎、赤芍进行专属性鉴别;采用高效液相色谱法对处方中君药何首乌中有效成分大黄素进行含量测定。色谱柱为Thermo Hypersil GOLD柱(250 mm×4.6 mm,5μm);流动相为甲醇-水(75∶25),流速为1.0 ml·min-1,检测波长为290 nm,柱温为室温,进样量为20μl。结果:3味药材的TLC特征斑点清晰,分离度好,阴性对照无干扰。大黄素在10~1 280μg·ml-1的浓度范围内呈良好线性关系,r=0.999 6。平均加样回收率为99.71%,RSD=2.16%(n=6)。结论:所建立的质量标准准确可靠、重复性良好,可用于乌参八味胶囊的质量控制。
Objective: To establish the quality standard of Wuwei Bawei capsule. Methods: The TLC, the Polygonum multiflorum, Chuanxiong, Radix Paeoniae Rubra specific identification; using high performance liquid chromatography on the prescription of Chinese medicine Polygonum multiflorum active ingredient emodin content determination. The column was Thermo Hypersil GOLD column (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (75:25), the flow rate was 1.0 ml · min-1, the detection wavelength was 290 nm, The sample volume is 20μl. Results: The three TLC herbs TLC features clear spots, good resolution, negative control without interference. Emodin in the 10 ~ 1 280μg · ml-1 concentration range showed a good linear relationship, r = 0.999 6. The average recovery was 99.71%, RSD = 2.16% (n = 6). Conclusion: The established quality standards are accurate and reproducible, and can be used for the quality control of Wuwei Bawei Capsule.