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在培养食用菌母种时,人们通常沿用玻璃试管琼脂斜面培育方法,管口用棉塞封闭.这种方法有不少优点,但也有不足之处,如PDA所培养的母种基质易干涸、液化,存放过程中易遭杂菌污染,菌丝易老化,转接次数多.为此笔者从1991年起开始试用盐水瓶培育平菇母种,取得理想的效果.现将具体方法介绍如下:1 准备材料①取100mL的盐水瓶,清洗后用0.1%新洁尔灭液冲洗,橡皮塞也同时冲洗.②取2mm口径的硬质塑料管6cm,在酒精灯上于2.5cm处弯成90°直角,在短段用刀削成斜面.③培养料为阔叶木屑和木粒(3～5mm)各半,玉米粉10%,石膏1%,石灰、酵母粉各2%,磷酸二氢钾、硫酸镁各0.1%,水65%.
In the cultivation of edible fungus mother, people usually follow the glass tube agar slant breeding method, the mouth with tampons closed.This method has many advantages, but there are also shortcomings, such as PDA culture of the mother substrate easy to dry up, Liquefaction, storage easy to be contaminated by bacteria, mycelium easy to aging, transfer times and more.To this end, the author started trial salt water bottle in 1991, mushroom mother, and obtain the desired effect.Will introduce the specific methods are as follows: 1 Prepare the material ① Take 100mL saline bottle, rinse with 0.1% benzalkonium bromide solution after rinsing, and rinse the rubber stopper at the same time. ② Take 2mm diameter hard plastic tube 6cm and bend it to 90 ° right angle at 2.5cm on the alcohol lamp, In the short segment with a knife into a bevel .③ training materials for hardwood and wood (3 ~ 5mm) half, corn flour 10%, gypsum 1%, lime, yeast extract 2%, potassium dihydrogen phosphate, sulfuric acid Magnesium 0.1%, water 65%.