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目的:研究凋亡素2配体(Apo-2 ligand,Apo-2L),或称肿瘤坏死因子相关凋亡诱导配体(TNF-related apoptosis-inducing lig-and,TRAIL)在体外对人肺腺癌A549细胞系的放射增敏作用的研究。方法:MTT法检测Apo-2L单药或与放射线联合对腺癌A549细胞的抑制率,将细胞分为4组,对照组、Apo-2L组、Apo-2L+放射照射组、单纯照射组,200ng/ml、286 ng/ml的Apo-2L作用24小时后给予放射照射,照射剂量分别为:(1Gy、1.4 Gy、1.8Gy、2 Gy、3 Gy),然后进行流式细胞仪分析照射后24h各组细胞凋亡率变化。结果:MTT结果显示,腺癌A549细胞的抑制率与Apo-2L的浓度成正相关,凋亡素2配体作用24h后IC50为286ng/ml.流式细胞仪分析显示286ng/ml的Apo-2L处理24h后,细胞凋亡率从(6.68)%上升至(50)%,照射后24h Apo-2L+照射组凋亡率明显升高,为72.790%,对照组0.1185%,Apo-2L组50%,单纯照射组51.5067%。结论:Apo-2L在体外对腺癌A549细胞有抑制增殖和促进凋亡作用,并且Apo-2L联合放射线可以明显提高腺癌A549细胞的凋亡率。
Objective: To investigate the effect of Apo-2 ligand (Apo-2L), or TNF-related apoptosis-inducing ligand (TRAIL) Radiosensitization of A549 cell line. Methods: The inhibitory rate of Apo-2L alone or combined with radiation on adenocarcinoma A549 cells was detected by MTT assay. The cells were divided into 4 groups: control group, Apo-2L group, Apo-2L + irradiation group, 24 hours after exposure to 286 ng / ml of Apo-2L for 24 hours, the irradiation doses were (1Gy, 1.4 Gy, 1.8 Gy, 2 Gy, 3 Gy), and then analyzed by flow cytometry 24h The apoptosis rate of each group changed. Results: The results of MTT showed that the inhibitory rate of A549 cells was positively correlated with the concentration of Apo-2L and the IC50 was 286ng / ml 24h after Apoptosis-2 ligand treatment.Flow cytometry analysis showed that 286ng / ml of Apo-2L The apoptotic rate increased from (6.68)% to (50%) at 24 h after treatment, and the apoptotic rate in Apo-2L + irradiation group was significantly higher at 72.790%, 0.1185% in control group and 50% in Apo-2L group , Simple irradiation group 51.5067%. CONCLUSION: Apo-2L can inhibit proliferation and promote apoptosis of A549 adenocarcinoma cells in vitro, and Apo-2L combined with radiation can significantly increase the apoptosis rate of A549 cells.