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目的:探讨脱氧核糖核酸酶(IDNA酶I)基因多态性与汉族人群不稳定性心绞痛(unstable angina pectoris,UAP)易感性的关系。方法:以196例UAP患者为病例组,排除冠心病的297例体检者为对照组,应用PCR及PCR-限制性片段长度多态(PCR-RFLP)分析DNA酶I基因8外显子单核苷酸多态位点A2317G及4内含子56bp可变串联重复序列(HumDN1)多态性;协方差分析A2317G、HumDN1各基因型与UAP患者血脂的关系,将年龄、性别、高血压、糖尿病及吸烟作为协变量;x2检验分析UAP患者冠脉血管病变支数与DNA酶I基因型的关系。结果:UAP组与对照组A2317G、HumDN1各基因型及等位基因分布无明显统计学差异(P>0.05),两组DNA酶I单体型分布亦无差异。UAP患者DNA酶I各基因型血脂水平、冠脉血管病变支数的差异无明显统计学意义,所有P值均>0.05。结论:DNA酶I基因多态性与中国汉族人群不稳定心绞痛及其血脂水平无明显相关性。
Objective: To investigate the relationship between polymorphism of DNA polymerase (IDNA) gene and susceptibility to unstable angina pectoris (UAP) in Han population. Methods: A total of 196 patients with UAP as the case group and 297 patients without coronary heart disease as the control group were analyzed by PCR and restriction fragment length polymorphism (PCR-RFLP) A2317G polymorphism and intron 56bp variable tandem repeats (HumDN1) polymorphism. The relationship between A2317G, HumDN1 genotypes and UAP patients was analyzed by covariance. Age, sex, hypertension, diabetes mellitus And smoking as covariates; x2 test analysis UAP patients with coronary artery lesions and DNase I genotype relationship. Results: The genotype and allele distribution of A2317G and HumDN1 in UAP group and control group were not significantly different (P> 0.05). There was no difference in the distribution of DNase I haplotype between the two groups. UAP patients with DNase I genotype lipid levels, coronary artery lesions count was no statistically significant difference, all P values> 0.05. Conclusion: DNase I gene polymorphism has no significant correlation with unstable angina pectoris and blood lipid levels in Chinese Han population.