正交试验法优选紫山药花青素提取工艺研究

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目的:采用正交试验法优化紫山药中花青素的提取工艺。方法:采用加热提取法,以花青素含量为评价指标,采用单因素试验及Ln 9(3n 4)正交试验考察固液比、提取温度、提取时间、盐酸浓度因素对花青素含量的影响。n 结果:影响提取工艺的因素依次为:料液比>温度>盐酸浓度>提取时间。紫山药中花青素的最佳提取工艺为:在料液比1∶10条件下,于2% HCl-40%乙醇溶剂中60 ℃提取40 min。结论:优化的提取工艺简单、合理、可行,可用于紫山药中花青素的提取。“,”Objective:To optimize the extraction technic of anthocyanins from Purple n Dioscorea alata L.by orthogonal test method.n Methods:To use heating extraction method and take anthocyanin content as the evaluation index, to investigate the effects of solid-liquid ratio, extraction temperature, extraction time and hydrochloric acid concentration on anthocyanin content with single factor test and Ln 9(3n 4) orthogonal test.n Results:The factors affecting the extraction process were as follows: solid liquid ratio > temperature > hydrochloric acid concentration > extraction time. The optimum extraction parameters of anthocyanins in purple n Dioscorea alata L. were as follows: 60 ℃, 2% HCl-40% ethanol, extract for 40 min, the solid-liquid ratio is 1:10.n Conclusion:The optimized extraction process is simple, reasonable and feasible, which can be used for the extraction of anthocyanins from purple n Dioscorea alata L..n
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