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业已证实,造血微环境在调节造血干细胞及其子代的增殖、分化中起着重要作用。同时,关于某些体液因子在应激和再生条件下对造血的影响亦有阐述。本文利用脾器官培养体系,就造血微环境及体液因子对造血再生的调节作用进行了研究。实验将800拉德全身照射并移植10~7同系骨髓细胞后24小时的C57BL小鼠脾脏移出,放在含20%胎牛血清的16毫升EMEM培养浓中,在含有10%CO_2潮湿空气中,于37℃条件下培养,在培养后的一定时间,用琼脂法测定脾造血祖细胞(CFU)的再生情况。结果表明,将照射过的脾脏作培养时,其微环境细胞在
It has been confirmed that the hematopoietic microenvironment plays an important role in the regulation of the proliferation and differentiation of hematopoietic stem cells and their offspring. At the same time, the influence of some humoral factors on hematopoiesis under stress and regeneration conditions is also elaborated. In this paper, the use of spleen organ culture system, hematopoietic microenvironment and humoral factors regulate the role of hematopoietic regeneration were studied. Experiments The C57BL mice spleen 24 hours after transplanting 10 to 7 myeloid cells with whole-body irradiation of 800 rads was removed and placed in 16 ml EMEM culture medium containing 20% fetal bovine serum in humidified air containing 10% CO 2, The cells were cultured at 37 ° C and the regeneration of spleen hematopoietic progenitor cells (CFU) was determined by agar method at a certain time after culture. The results showed that the irradiated spleen was cultured in the microenvironment when the cells were in