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观察丹参酸乙对肝细胞增殖及胶原生成率的影响，探讨其抗肝纤维化作用机制。方法灌流法分离肝细胞进行体外原代培养，［３Ｈ］－ＴｄＲ掺入检测细胞增殖率，［３Ｈ］－脯氨醉掺入、胶原酶消化法检测胶原生成率，凝胶法测定胶原酶活性。结果丹参酸乙可显著促进肝细胞增殖，提高细胞内总蛋白生成量，降低胶原蛋白生成，抑制胶原生成率；提高细胞分泌的间质型脐原酶活性，抑制细胞外的胶原生成率，均以１０－６ｍｏｌ／Ｌ浓度最好（与对照组比较，Ｐ值＜０．０５）。结论丹参酸乙可促进肝细胞增殖，抑制细胞的胶原生成。在抗肝纤维化治疗中具有特殊的意义。
To observe the effect of Salvia miltiorrhiza B on hepatocyte proliferation and collagen production, and to explore its anti-hepatic fibrosis mechanism. Methods The primary culture of hepatocytes was isolated by perfusion method. The proliferation rate of cells was detected by [3H] -TdR incorporation, the incorporation of [3H] -hydrochloride and collagenase digestion, the collagenase activity . Results Salvia acid B could significantly promote the proliferation of hepatocytes, increase the intracellular total protein production, reduce collagen production, inhibit the collagen production rate, increase the secretion of interstitial umbilical cofactor, and inhibit the extracellular collagen production rate The best concentration of 10-6mol / L (compared with the control group, P value <0.05). Conclusion Salvia acid B can promote the proliferation of hepatocytes and inhibit the collagen production. In the anti-fibrosis treatment has a special significance.