论文部分内容阅读
目的研究瘤内注射的树突状细胞(DC)的迁移及在肿瘤局部的作用。方法建立大鼠C6脑胶质瘤颅内肿瘤模型,脂质体介导的增强型绿色荧光蛋白(EGFP)基因转染DC细胞,瘤内注射DC后取大鼠同侧的淋巴结在荧光显微镜下检测,并用流式细胞仪检测肿瘤周围组织CD4+ T和CD8+T细胞的浸润。结果同侧的颈深部淋巴结可见EGFP表达的DC,对侧淋巴结未见到。 DC治疗组证实有CD4+T和CD8+T明显浸润(CD4+T 8．19％,CD8+T11．05％),RPMI 1640治疗组CD4+T0．95％和CD8+T1．17％,生理盐水对照组CD4+T0．62％和CD8+T0．77％。结论瘤内注射的DC不仅能够迁移,而且有效地引起肿瘤局部的T细胞显著浸润,为瘤内注射DC细胞提供了依据。
Objective To study the migration of dendritic cells (DCs) injected intratumorally and their role in tumor localization. Methods To establish an intracranial tumor model of C6 glioma in rats. Liposome-mediated enhanced green fluorescent protein (EGFP) gene was transfected into DCs. After intratumoral injection of DCs, ipsilateral lymph nodes of rats were examined under a fluorescence microscope The infiltration of CD4 + T and CD8 + T cells in the surrounding tissues was detected by flow cytometry. Results The ipsilateral cervical deep lymph nodes showed EGFP expression of DC, contralateral lymph nodes not seen. CD4 + T and CD8 + T were significantly increased in DC group (CD4 + T 8.19%, CD8 + T11.05%), CD4 + T0.95% and CD8 + T1.17% in RPMI 1640 group, Saline control CD4 + T0.62% and CD8 + T0.77%. Conclusion Intratumoral injection of DC can not only migrate, but also effectively induce significant infiltration of T cells in the tumor area, providing a basis for intratumoral injection of DC cells.