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目的 探讨新型心肌灌注显像(MPI)药物2-叔丁基-4-氯-5-[4-(2-氟-18F-乙氧基甲基)苯基甲氧基]-3(2H)-哒嗪酮(18F-Flurpiridaz)在小型猪体内的生物分布及定量分析价值,并与13N-NH3·H2O进行对比.方法 取10只巴马小型猪,分为正常组与心肌梗死组,每组各5只.正常组不行处理,心肌梗死组采用外科开胸结扎冠状动脉的方式建模.2组均先行13N-NH3·H2O显像,后行18F-Flurpiridaz显像,2种显像至少间隔40 min,注射剂量均为185～370 MBq.正常组另行18F-Flurpiridaz全身显像,观察18F-Flurpiridaz的生物分布,计算心肌与邻近组织或器官18F-Flurpiridaz的放射性摄取比值.比较正常组2种显像图像质量及静息心肌血流量(rMBF);比较心肌梗死组2种显像图像质量及静息总评分(SRS)、心肌梗死面积百分比、总灌注缺损(TPD)、左心室射血分数(LVEF)等常用心功能评价参数.采用配对t检验分析数据.结果 全身显像示正常组小型猪18F-Flurpiridaz MPI心肌显影清晰,注射后2h内始终保持高放射性摄取,左心室心肌与心血池、肺和肝的放射性计数比值高(5.19～ 12.87、4.17～ 50.51、2.08～6.92).2组动物的18F-Flurpiridaz MPI图像全部(10/10)质量优秀.18F-Flurpiridaz与13N-NH3·H2O显像测得的左心室不同区域rMBF(ml·g-1·min-1)差异均无统计学意义(左前降支:0.98±0.06和0.92±0.13;左回旋支:0.98±0.05和0.88±0.12;右冠状动脉:0.95±0.07和0.88±0.15;左心室:0.96±0.07和0.90±0.13;t值:-1.70～-0.90,均P＞0.05);两者所得的心功能参数差异亦无统计学意义[SRS:10.6±4.1和9.2±4.6;心肌梗死面积百分比:(15.2±9.0)％和(12.6±6.6)％;TPD:(11.6±6.3)％和(9.6±3.9)％;LVEF:(68.6±11.1)％和(71.4±11.3)％;t值:-2.33～2.75,均P＞0.05].结论 与13N-NH3·H2O相比,18F-Flurpiridaz MPI图像质量好、心功能参数测定准确,具备心肌血流定量的潜力,是一种具有广阔应用前景的正电子MPI药物.“,”Objective To explore the biodistribution and quantitative value of 18F-Flurpiridaz in mini-swine,and compare with 13N-NH3 · H2O.Methods Ten Bama mini-swine were divided into normal group and myocardial infarction group (n=5 in each group).Normal group was not treated and myocardial infarction group was modeled by thoracotomy and coronary artery ligation.Both groups were preceded by 13N-NH3 · H2O imaging,followed by 18F-Flurpiridaz imaging (time interval ＞40 min).Injection dosage of 2 tracers was the same (185-370 MBq).18F-Flurpiridaz whole-body PET/CT imaging was also performed in normal group.Biological distribution of 18F-Flurpiridaz was observed,and the ratio of radioactive uptake of 18F-Flurpiridaz between myocardium and adjacent tissues or organs was calculated.Image quality score and rest myocardial blood flow (rMBF) of 2 imaging tracers in normal group were measured and compared.MPI image quality score,cardiac function parameters such as summed rest score (SRS),myocardial infarction area percentage,total perfusion defect (TPD),and left ventricular ejection fraction (LVEF) of 2 imaging tracers were compared in myocardial infarction group.Data was analyzed by paired t test.Results In normal group,18F-Flurpiridaz in the myocardium was clearly observed,with high radioactive uptake maintaining within 2 h postinjection.The radioactivity count ratios of left ventricular myocardium to cardiac pool,the lungs and liver were high (5.19-12.87,4.17-50.51,2.08-6.92).The quality of 18F-Flurpiridaz MPI images in both groups was excellent (10/10).The rMBF (ml · g-1 · min-1) in different regions of left ventricle measured by 18F-Flurpiridaz and 13N-NH3 · H2O imaging were not significantly different (left anterior descending:0.98±0.06 vs 0.92±0.13;left circumflex:0.98±0.05 vs 0.88±0.12;right coronary artery:0.95±0.07 vs 0.88±0.15;left ventricle:0.96±0.07 vs 0.90±0.13;t values:from-1.70 to-0.90,all P＞0.05).There was no significant difference in SRS,myocardial infarction area percentage,TPD,rMBF or LVEF between 18F-Flurpiridaz and 13N-NH3 · H2O (SRS:10.6±4.1 vs 9.2±4.6;myocardial infarction area percentage:(15.2±9.0)％ vs (12.6±6.6)％;TPD:(11.6±6.3)％ vs (9.6±3.9)％;LVEF:(68.6±11.1)％ vs (71.4±11.3)％;t values:-2.33-2.75,all P＞0.05).Conclusions Comparing with 13N-NH3 · H2O,18F-Flurpiridaz has the advantages of good MPI image quality,accurate measurement of cardiac function parameters and quantitative potential of myocardial blood flow,which make it as a promising positron myocardial perfusion imaging agent.