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为研究水稻细胞质雄性不育的分子基础,我们提取纯化了红莲型水稻丛广41A 和丛广41B 的线粒体DNA,采用限制性内切酶酶切片段长度多态性(RFLP) 方法比较了红莲型不育系和保持系线粒体DNA酶切图谱,发现两者之间存在一定的差异,部分支持了细胞质雄性不育与线粒体DNA 有关的结论
In order to study the molecular basis of cytoplasmic male sterility in rice, we extracted and purified the mitochondrial DNA of Honglian rice variety Congguang 41A and Congguang 41B, and compared the results of RFLP with the restriction endonuclease restriction fragment length polymorphism (RFLP) There were some differences between the two types of CMS lines and maintainer lines in mitochondrial DNA digestion, which partially supported the conclusion that cytoplasmic male sterility is related to mitochondrial DNA