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目的和方法：对大鼠肺微血管内皮细胞（ＰＭＶＥＣ）培养、鉴定。观察了低浓度Ｈ２Ｏ２（０～１００μＭ）对体外培养的ＰＭＶＥＣ的迟发性损伤效应。结果：ＭＴＴ法发现Ｈ２Ｏ２对ＰＭＶＥＣ的增殖代谢活性具有剂量依赖性抑制作用，而且当Ｈ２Ｏ２去除后，此作用仍可进一步发展。形态观察、流式细胞仪ＤＮＡ测定、３’末端标记ＤＮＡ降解片断原位检测（ＴＵＮＥＬ）均表明，细胞凋亡是Ｈ２Ｏ２对ＰＭＶＥＣ迟发性损伤效应的一种重要形式。
Objective and Methods: The rat pulmonary microvascular endothelial cells (PMVEC) were cultured and identified. The delayed injury of PMVEC cultured in vitro was observed with low concentration of H2O2 (0 ~ 100μM). Results: MTT assay found that H2O2 had a dose-dependent inhibitory effect on the proliferation and metabolism of PMVEC, and this effect could be further developed when H2O2 was removed. Morphological observation, flow cytometry DNA assay, and 3’-end labeled DNA degradation fragment in situ assay (TUNEL) showed that apoptosis was an important form of delayed damage of PMVEC by H2O2.