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目的探讨线粒体转录终止因子(MTERFs)蛋白家族在组织和细胞中的表达以及在神经毒素MPTP/MPP+诱导下的表达变化。方法取正常C57BL小黑鼠的脑、肝、肾组织,以及人神经母细胞瘤细胞SH-SY5Y、人心肌细胞HCM、人肝细胞L-02,检测MTERFs的蛋白表达情况;通过MPTP/MPP+构建帕金森病(PD)动物/细胞模型,检测MTERFs的蛋白表达变化情况。结果 MTERFs在小鼠脑中和SH-SY5Y细胞中蛋白表达水平较高;MPTP/MPP+诱导的PD模型中,MTERF3蛋白表达水平明显降低。结论在小鼠脑和SH-SY5Y细胞中,MTERFs表达较高;MPTP/MPP+诱导下MTERF3蛋白表达明显降低,影响了线粒体转录水平,这可能参与了帕金森病发生的过程。
Objective To investigate the expression of mitochondrial transcriptional termination factor (MTERFs) protein family in tissues and cells and the expression change induced by neurotoxin MPTP / MPP +. Methods The brain, liver and kidney tissues of normal C57BL mice and the SH-SY5Y, human cardiomyocytic HCM and human hepatocyte L-02 of human neuroblastoma cells were detected. The protein expression of MTERFs was detected by MPTP / MPP + Parkinson’s disease (PD) animal / cell model to detect changes in protein expression of MTERFs. Results The expression of MTERFs in mouse brain and SH-SY5Y cells was higher. The expression of MTERF3 protein in MPTP / MPP + -induced PD model was significantly decreased. Conclusions MTERFs are highly expressed in mouse brain and SH-SY5Y cells. The expression of MTERF3 is significantly down-regulated under MPTP / MPP + stimulation, which may affect the transcription of mitochondria. This may be involved in the pathogenesis of Parkinson’s disease.