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目的:探讨猪与人皮肤抗原性的差异。方法:人和小猪全厚正常皮肤作冰冻切片,用抗人皮肤组织相关抗原(HLA-ABC,HLA-DR,波形蛋白,结蛋白,肌动蛋白,Ⅷ因子相关抗原,细胞角蛋白)、细胞因子及其受体和胞外基质(透明质酸,纤维结合蛋白,层粘连蛋白,Ⅳ胶原,雌二醇-17β和 testosterone)及Ⅰ/Ⅲ/Ⅶ型胶原等抗原成分的单克隆或多克隆抗体进行免疫组化染色,比较猪与人皮肤组织抗原性异同。结果:在猪皮肤组织可检测到Ⅰ/Ⅲ/Ⅳ/Ⅶ型胶原和透明质酸,纤维结合蛋白,层粘连蛋白,FⅧ因子相关抗原,细胞角蛋白,IL-6,IL-8,雌二醇-17β,testosterone 和 ICAM-1;多克隆抗体阳检率明显高于单克隆抗体;其中细胞外基质成分和分子量较小的细胞因子,以及结构或功能原始的蛋白分子在猪与人皮肤有较好的同源性,而进化较大的蛋白分子(如细胞因子或激素的受体和免疫细胞分型的 CD 分子等)则多为阴性,显示抗体-抗原结合反应的特异性及其种族差异。结论:猪与人皮肤组织抗原之间存在很大的差异,猪血管内皮细胞表达人 ICAM-1,提示二者粘附分子可能存在物种间的交叉,可能与异种皮肤移植难以存活有关;本研究为异种脱细胞真皮基质的临床应用提供了有用的资料。
Objective: To explore the differences of skin antigenicity between pigs and humans. Methods: Frozen sections of human and pig full thickness normal skin were harvested with anti-human dermal tissue-associated antigen (HLA-ABC, HLA-DR, vimentin, desmin, actin, factor Ⅷ related antigen, cytokeratin) Cytokines and their receptors and extracellular matrix (hyaluronic acid, fibronectin, laminin, Ⅳ collagen, estradiol-17β and testosterone) and Ⅰ / Ⅲ / Ⅶ collagen and other antigenic components of monoclonal or more Clonal antibodies were immunohistochemically stained to compare antigenicity between pigs and human skin tissues. Results: Type Ⅰ / Ⅲ / Ⅳ / Ⅶ collagen and hyaluronic acid, fibronectin, laminin, factor Ⅷ related antigen, cytokeratin, IL-6, IL- Alcohol-17β, testosterone and ICAM-1. The positive rate of polyclonal antibody was significantly higher than that of monoclonal antibody. Among them, extracellular matrix components and small molecular weight cytokines, as well as structural or functional original protein molecules, Better homology, and more evolved protein molecules (such as cytokines or hormone receptors and immune cell types of CD molecules, etc.) are mostly negative, showing the specificity of the antibody - antigen binding reaction and race difference. CONCLUSION: There is a great difference between porcine and human dermal tissue antigens. The expression of human ICAM-1 in pig vascular endothelial cells suggests that there may exist inter-species crosses between the two adhesion molecules, which may be related to the difficult survival of xenograft skin graft. In this study The clinical application of xenogenic acellular dermal matrix provides useful information.