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利用甲基磺酸乙酯对常规粳稻‘秀水09’进行诱变处理,获得了3个黄叶突变体Yl-1,Yl-2和Yl-3,多代自交稳定遗传。突变体与野生型‘秀水09’相比,分蘖减少、植株变矮、苗期叶片表现明显的黄色,随着生长发育的进行,叶片逐渐返绿。遗传分析表明这3个突变体的黄叶性状均受1对隐性核基因控制。利用SSR分子标记,将突变基因Yl-1定位在第3号染色体RM282与RM6080之间的7.5 cM范围内,将突变基因Yl-2和Yl-3定位在第5号染色体分子标记5-43w与RM1237之间,遗传距离分别为2.0 cM和6.8 cM。
Three yellow-leaf mutants Yl-1, Yl-2 and Yl-3 were obtained by mutagenesis treatment of conventional japonica rice Xiushui 09 with ethyl methanesulfonate. Compared with the wild type ’Xiushui 09’, the mutant had fewer tillers, shorter plants, and yellow leaves at seedling stage. With the growth and development, the leaves gradually returned to green. Genetic analysis indicated that the yellow leaf traits of these three mutants were all controlled by one recessive nuclear gene. Using the SSR molecular marker, the mutant gene Yl-1 was located within 7.5 cM between RM282 and RM6080 on chromosome 3, and the mutant genes Yl-2 and Yl-3 were mapped on chromosome 5 to 5-43 w and RM1237, the genetic distance was 2.0 cM and 6.8 cM respectively.