从方法学角度介绍肺泡Ⅱ型上皮细胞的大多数分离措施、各种纯化方法、光学显微镜和电子显微镜鉴定的优缺点，酶学方法鉴定该类细胞的独到之处，原代细胞培养的条件选择及其注意事项 Most methods of isolation of alveolar type Ⅱ epithelial cells are introduced from the perspective of methodology. Various purification methods, the advantages and disadvantages of light microscopy and electron microscopy are identified. The uniqueness of this kind of cells is identified by enzymatic methods. The conditions of primary cell culture And its precautions