用 HPL C法分离了柴胡止血口服液中白芍的芍药甙 ,并测定其含量。分析条件为色谱柱 Phcnom enex ODS柱(5 μm,15 0× 4.6 m ml D) ,预柱 (Althle) ;流动相 :甲醇 -36 %醋酸 -异丙醇 -水 (2 5∶ 2∶ 2∶ 71) ;流速 :1.0 ml/ min;柱温 :室温 ;检测波长 :2 30 nm ;灵敏度 :0 .1A UF S。测定三批样品 ,加样回收率平均值为 97.5 8% ,RSD为 1.32 %。 HPL C method was used to separate Paeonia lactiflora from Radix Bupleuri Hemostasis Oral Liquid and determine its content. The analytical conditions were as follows: column Phnomon enex ODS column (5 μm, 15×4.6 m ml D), pre-column (Althle); mobile phase: methanol-36% acetic acid-isopropanol-water (25:2:2: 71) Flow rate: 1.0 ml/min; Column temperature: room temperature; Detection wavelength: 2 30 nm; Sensitivity: 0.1A UF S. Three batches of samples were determined. The average recovery of the sample was 97.58%, and the RSD was 1.32%.