对由湖北省某公证处封存的两份杂交稻材料进行ＤＮＡ、蛋白质指纹鉴定，首先采用业已建立的可特异鉴定以明恢６３为父本的杂交稻真、假的Ｐ１８引物进行ＲＡＰＤ分析，发现两份材料的叶片及种子ＤＮＡ在０．８ｋｂ扩增带上存在着有和无的区别；而所用的４２个水稻微卫星标记，发现５个水稻微卫星标记具有多态性；在此基础上，进一步对两样品的种子水溶性蛋白质毛细管电泳图谱进行比较，二者在出峰时间及峰值上存在差异。上述结果表明，两份材料并非同一杂交组合，但在遗传背景上存在较大的相似性。 The DNA and protein fingerprinting of two hybrid rice materials sealed by a notary office in Hubei Province were identified. First, by using RAPD analysis of the P18 primers that can specifically identify hybrid rice with Minghui63 as male parent, P18 primers were used for RAPD analysis. On the basis of the 42 rice microsatellite markers, five rice microsatellite markers were found to have polymorphism; on the basis of this, , Further compared the two samples of water-soluble protein capillary electrophoresis spectra, the two peaks in the peak time and there are differences. The above results show that the two materials are not the same hybrid combination, but there is a large similarity in the genetic background.