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运用荧光光谱、吸收光谱研究了Eu(Ⅲ)、Cu(Ⅱ)对灯盏花素(Breviscapinun,BR)与牛血清白蛋白(Bovine serum al-bumin,BSA)作用的扰动。金属离子存在时BR对BSA的猝灭机制未发生改变,通过计算获得了二者相互作用的KA、n及热力学参数。结果表明,Cu(Ⅱ)的存在使得BR与BSA二者(BR-BSA)结合常数、结合位点数均增大,二者的作用力并未发生变化;而Eu(Ⅲ)存在时,BR-BSA作用的结合常数、结合位点数均减小,BR-BSA之间的作用力由疏水作用变为范德华力和氢键。从离子架桥作用、金属离子与BR的竞争作用,热力学参数的变化、配位化合物的形成等方面分析了影响BR-BSA作用的因素。
The effects of Eu (Ⅲ) and Cu (Ⅱ) on the interaction of breviscapinun (BR) and bovine serum albumin (BSA) were studied by fluorescence and absorption spectroscopy. In the presence of metal ions, the quenching mechanism of BR on BSA did not change, and the KA, n and thermodynamic parameters of the interaction between BR and BSA were obtained by calculation. The results showed that the binding force of BR-BSA and the number of binding sites increased with the presence of Cu (Ⅱ), and the force of the two did not change. However, in the presence of Eu (Ⅲ) The binding constants of BSA and the number of binding sites decreased, and the interaction between BR-BSA changed from hydrophobic to van der Waals forces and hydrogen bonding. The factors influencing the action of BR-BSA were analyzed from the aspects of ion bridging, the competition between metal ions and BR, the change of thermodynamic parameters and the formation of coordination compounds.