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目的探讨念珠菌在母婴间垂直传播的可能途径。方法采用分子生物学方法,对11对分离于分娩前母亲阴道分泌物及其新生儿口腔并经常规形态学和生化学鉴定为同种念珠菌的母婴分离株,再次进行大亚基rDNA的D1/D2区域DNA序列鉴定。采用电泳核型和随机扩增DNA多态性进行分型研究,以确定是否为同一来源的菌株。结果11对22株菌,其中8对母婴分离株的16株菌的DNA序列经DDBJGeneBank(登录号U45776)搜索与白念珠菌参考株Cal_Y12983具有100%的同源序列,被确认为白念珠菌;另2对的4株菌和1对的2株菌的DNA序列经DDBJGeneBank搜索与Cgra_Y65(U44808)和C.kruseiY-5396(U76347)具有100%的同源序列,分别被确认为光滑念珠菌和克柔念珠菌。电泳核型分析,每一对白念珠菌和克柔念珠菌的母婴分离株都具有完全相同的谱带。每一对光滑念珠菌的母婴分离株尽管产生1、2条不同谱带,但仍被认为是来源于同一个体,因为主要谱带是相同的。RAPDDNA带谱分析,对母婴分离株除1对2株菌的DNA谱带略有不同外,其余10对皆具有同样的DNA谱带。结论1111株婴儿株与其母亲株分子生物学特征完全相同,可认为是经母亲产道垂直传播所致。
Objective To explore the possible pathways of Candida in vertical transmission between mother and infant. Methods Using molecular biology method, 11 pairs of maternal and infant isolates isolated from vaginal secretions of mother and their newborns before delivery and identified by conventional morphological and biochemical methods as Candida albicans were re-performed with large subunit rDNA D1 / D2 region DNA sequence identification. Electrophoretic karyotypes and random amplified polymorphic DNA were used for typing to determine if they were from the same source. Results The DNA sequences of 16 isolates from 11 pairs of 22 isolates from 8 isolates of mother and infant were searched by DDBJGeneBank (Accession No. U45776), which had 100% homology with the reference strain Cal_Y12983 of Candida albicans, which was identified as Candida albicans ; The DNA sequences of the other two pairs of 4 strains and 1 pair of 2 strains were 100% homologous to Cgra_Y65 (U44808) and C. krusei Y-5396 (U76347) by DDBJGeneBank and were identified as Candida glabrata Candida krusei. Electrophoresis analysis of karyotypes showed that both maternal and infant isolates of Candida albicans and Candida krusei had identical bands. Each pair of Candida glabrata maternal and infant isolates is considered to be derived from the same individual, although producing 1,2 distinct bands because the major bands are the same. RAPD band spectrum analysis, except for one to two strains of the mother and baby isolates DNA bands slightly different, the other 10 pairs have the same DNA bands. Conclusion The 1111 strains of infants share the same molecular biological characteristics as their mother strains and may be thought to be caused by the vertical transmission of the mother’s birth canal.