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采用双向电泳-质谱技术筛选肝癌特异的血清蛋白标记物,以利于肝癌的早期诊断和治疗.肝癌、肝炎和正常三组各20例血清先采用超声、高丰度蛋白去除、脱盐预处理以优化双向电泳,图像分析三组血清图谱寻找差异点,基质辅助激光解吸飞行时间质谱对差异点进行鉴定.结果显示,通过样品预处理,血清上样体积平均增加3～4倍,参考胶点数由218个增至332个,白蛋白和免疫球蛋白明显减弱,水平条带明显减少.图谱比较所得37个差异点,经鉴定为7种蛋白.与正常组比较,转铁蛋白、甲状腺素运载蛋白在肝炎和肝癌组低表达,α-1抗胰蛋白酶、凝聚素、铜蓝蛋白、触珠蛋白在肝炎和肝癌组均高表达.α-1抗胰蛋白酶在肝癌组较肝炎组高表达,而热休克蛋白27只在肝癌组表达.上述结果提示,双向电泳-质谱技术可发现肝癌发生发展过程中血清蛋白表达谱质或量的变化,从而为肝癌的早期诊断及治疗奠定基础.
Two-dimensional electrophoresis-mass spectrometry was used to screen the liver cancer-specific serum protein markers in order to facilitate the early diagnosis and treatment of hepatocellular carcinoma.Hepatocarcinoma, hepatitis and normal three groups of 20 cases of each serum first ultrasound, high abundance protein removal, desalination pretreatment to optimize Two-dimensional electrophoresis and image analysis were used to identify the difference of serum profiles and the matrix-assisted laser desorption / time of flight mass spectrometry to identify the difference points.The results showed that the sample volume increased by 3 ~ 4 times on average and the reference glue points increased from 218 Increased to 332, albumin and immunoglobulin were significantly weakened, the level of the band was significantly reduced.Comparison of the 37 spots obtained by the map, identified as seven kinds of protein.Compared with the normal group, transferrin, thyroxin carrier protein in Hepatitis and hepatocarcinoma group had low expression of α-1 antitrypsin, lectin, ceruloplasmin and haptoglobin in both hepatitis and hepatocellular carcinoma groups.α-1 antitrypsin was highly expressed in hepatocellular carcinoma group than in hepatitis group, Shock protein 27 was only expressed in HCC group.The above results suggest that the two-dimensional electrophoresis-mass spectrometry can detect changes in the content or amount of serum protein during the development of HCC, Diagnosis and treatment of the foundation.